Linearizing the Calibration Curve in Determination of Sulfate by the Methylthyrnol Blue Method
نویسنده
چکیده
The linearization of the callbratlon curve of the automated methylthymol blue (MTB) procedure for the determination of sulfates is described. Thls is accomplished by altering the barium-to-MTB molar ratio of the barium-MTB reagent used. Because of the low purlty of the commerclal dye, bariumto-MTB molar ratios of 0.9:l or lower are usually needed for obtaining linear callbratlon curves. A llnear relatlon between absorbance and sulfate concentration with a correlatlon coefficient of 0.9995 or better is obtained. The relative standard deviation among repllcates, in the range of 0 to 100 pg/ml, is 1.4 to 0.4%. The sensitivity for this range is 4 pg/ml SO4*-.
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